Cell viability is the measure of the proportion of living cells in a population. Used to determine the number of living or dead cells in a total sample so the experiment can be optimized if required. It does not differentiate between dividing and non-dividing cells. Viable cells = x Dead cells = y Total cells […]
Category: Biology
Biology is the study of all living things. In this field of science, we study the functioning of the body, different cells, and organisms.This field is further divided into many other fields such as Bioinformatics, Biochemistry, biotechnology, microbiology.
Why Nature choose Phosphorous and not any other element of Periodic Table?
Phosphorous is an element of the periodic table with the atomic number 15. In nature, it is commonly present in the form of phosphates. It plays a crucial role in many biological processes. It would not be wrong to say that it will be difficult to create life without Phosphates.
Agarose gel Electrophoresis: Principle and procedure
what is electrophoresis? Electrophoresis is the movement of particles/molecules under a spatially uniform electric field in a buffer. When charged molecules are placed in an electric field, they migrate toward the +ve or -ve pole according to the charge in the molecule. The rate of migration of the molecules depends on the strength of the […]
ANTIGEN PROPERTIES AND TYPES
What is an antigen? An antigen is defined as any substance which can stimulate our body to make an immune response against that substance. Example Bacteria, Viruses, Chemicals PROPERTIES OF ANTIGENS Antigens are foreign in nature. Antigens are chemical (Proteins, polysaccharides) Antigens have a minimum molecular mass of 5000 Da. The complex structure of an […]
Biosafety Levels
According to the W.H.O. (World Health Organization), ” Biosafety is a strategic and integrated approach to analyzing and managing relevant risks to human, animal and plant life and health and associated risks for the environment. Biosafety is based on the link between different sectors and the potential of the hazards to spread between sectors. Biosafety […]
Types Of PCR (polymerase chain reaction )
The polymerase chain reaction is a biological technology to produce an amplified number of DNA copies of a sequence. Three primary steps involved are denaturation, annealing, and extension. PCR techniques have a lot of applications in plant biology, diagnosis of influenza- human brucellosis Salmonellae, cloning purpose, in the field of Dentistry, microbiology, forensic science, etc., […]
Cell signaling
Cell signaling is a process for the cells to communicate with each other within the multicellular organism. The cell can either self-communicate or communicate to distance(other cells). A signal is a molecule that binds to a specific receptor and stimulates an action/mechanism. There are various possible types by which cells can communicate with each other. […]
How to count amino acid in a protein sequence using Python program
In this article, we are going to learn how python can be useful in finding amino acid in a given protein sequence. Before reading this article you must know about FASTA format and single letter code for the amino acid. (Click here FASTA format) Here, we will learn how can we find the total length […]
CARBOHYDRATES
Carbohydrates are the biomolecules consisting of aldehyde and ketone with multiple -OH groups. Carbohydrates are the main source of energy are made of 3 elements Carbon, Hydrogen, and Oxygen some of them also contain phosphorus or Sulphur. Classification of carbohydrates Carbohydrates are mainly classified as : Monosaccharides Oligosaccharides Polysaccharides The word saccharide derived from the […]
WATER and pH
WATER A water molecule is an irregular and irregular slightly skewed tetrahedron with an oxygen atom at its center. Water is a solvent for a wide range of organic molecules. Water molecules can dissociate into hydroxide ions(OH-) and protons (H+). Structure of water Water is a dipole molecule. Water molecules have inter and intra linked […]