Fasta format and Sequence comparison

What is fasta sequence? Fasta is a format used to represent nucleotide sequences or protein/peptide sequences. In the fasta format, the sequences are represented with their single letter code. E.g., for nucleotides A, T, G, C, U For proteins: A, M, D, etc. The fasta sequence of any nucleotide or peptide begins with a single […]

BACTERIAL GROWTH

Bacterial growth is an increase in the cell in cell mass and cell number. Bacterial Growth Curve:  under favorable conditions bacterial population doubles at regular intervals. When a fresh medium is inoculated with cells and the bacterial population growth is monitored over some time, plotting data between growth and time leads to a typical bacterial […]

Central Dogma of Molecular Biology

The central dogma is the flow of information (DNA—RNA–Protein). The process of central dogma explains how the DNA is converted into a functional product. It was first proposed by Francis Crick (1957) and published (1958). Central Dogma also confirms that our DNA contains all the information that is required to make proteins. A gene is […]

PLASMIDS

The plasmid is the extrachromosomal DNA that can independently replicate. Most commonly plasmids are found in bacteria as double-stranded circular DNA. Sometime plasmid can be found in multicellular organisms. Plasmid classification: Conjugative and Non-conjugative Plasmid Conjugative plasmids are characterized by the ability to promote sexual conjugation between bacterial cells. Non-conjugative plasmid can’t make sexual conjugation. […]

Introduction to biomolecules

Biomolecules are the biochemical molecule present in organisms that are essential in different biological processes. E.g., carbohydrate, fatty acids | cell division, replication Biomolecules include macromolecules such as lipids and carbohydrates, protein. Biomolecules are generally endogenous and exogenous. Endogenous: produced inside the organism. Exogenous: need to be taken such as vitamins. Most of the biomolecules […]

Gram staining

Gram staining is a method used to differentiate two groups of bacteria. Based on the cell wall. Developed by Hans Christian Gram in the 19th This method is used to identify gram +ve gram –ve After performing staining the Gram-positive bacterium stain violet and Gram-negative bacterium stain pink. This technique of staining can’t define bacteria, […]

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